By D.S. Mitrinovic, E.S. Barnes, J.R.M. Radok
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5] F = IPinh IPo where IPinh is the induction period in the presence of an inhibitor, and IPo is the induction period of the non-inhibited system. 6] ORR = Winh Wo where Winh is the oxidation rate in the presence of an inhibitor, and Wo is the rate of non-inhibited oxidation. F is a measure of the effectiveness and ORR is an inverse measure of the strength of the antioxidant. 6 Reaction products from a hindered phenol (BHT) during autoxidation. Inhibiting oxidation 35 the ORR, the stronger the inhibitor.
13. Badings H T, ‘Cold storage defects in butter and their relation to the autoxidation of unsaturated fatty acids’, Ned. , 1970 24 147–256. 14. Ellis R, Gaddis A M, Currie G T and Powell S L, ‘Carbonyls in oxidising fat: XII. The isolation of free aldehydes from autoxidised triolein, trilinolein, and trilinolenin’, J. Am. Oil Chem. , 1968 45 552–59. 15. ), Volume IX, American Association of Cereal Chemists, St Paul, MN, 1988, 161–215. V. 1 Critical points of oxidation Oxidation is generally treated as the most frequently occurring form of lipid deterioration, which leads to the development of rancidity, off-flavour compounds, polymerisation, reversion, and other reactions causing reduction of shelf life and nutritive value of the food product.
Soy isozyme 3 is similar to isozyme 2, but its activity is inhibited by calcium ions, whereas lipoxygenase 2 is stimulated by the metal. Lipoxygenase 4 is very similar to isozyme 3, but can be separated by gel chromatography or electrophoresis. Lipoxygenase isozymes are commonly classified as type 1, which have an optimum pH in the alkaline region and are specific for free fatty acids, and type 2, which has optimum activity at neutral pH and causes co-oxidation of carotenoids. The ability of lipoxygenase type 2 to bleach carotenoids has found practical application in the addition of soya flour to wheat flour in order to bleach the flour in the manufacture of white bread.