By Perpetua M. Muganda
This distinctive quantity presents a unmarried, important reference resource for tactics that definitively determine and correctly quantify apoptosis. The e-book starts off with universal equipment applied to discover and quantitate apoptosis, in addition to apoptosis signaling pathways in toxicological and different similar learn. It keeps with multi-parametric and phased apoptosis assays for detecting early and past due apoptosis or distinguishing apoptosis from necrosis and autophagy. next chapters concentrate on contemporary advances in genuine time and high-throughput assays that become aware of and quantitate apoptosis and apoptosis signaling pathways. ultimate chapters specialise in fresh advancements in preclinical anticancer therapeutics concentrating on apoptosis. Written for the Methods in Pharmacology and Toxicology sequence, chapters characteristic step by step descriptions of the methodologies, in addition to specialist tips and implementation advice.
Vital and authoritative, Apoptosis tools in Toxicology serves amateur scientists in addition to specialists, using a variety of tools from universal laboratory gear to high-end pricey and automatic equipment in a position to acting genuine time apoptotic measurements.
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Extra info for Apoptosis Methods in Toxicology
Camptothecin) that are effective by inducing intrinsic mediated apoptosis. Together these results showcase how our assay allows for an unprecedented detection of both caspase 3 and caspase 8 activity in a high throughput format. From a technical aspect, our assay is superior in sensitivity, simplicity, stability, convenience, and robustness when compared to the other described apoptosis assays. Also, our methodology has been uniquely adapted to eliminate background autofluorescence associated with cell culture media; this helps to achieve an improved signal-to-noise ratio and avoids high background subtractions which can complicate FRET calculations.
In this regard, small molecule caspase ligands offer an attractive alternative. The most prominent examples exist within the isatin sulfonamide moiety family. Radiolabeled isatin sulfonamides, such as [18F]WC-II-89 [120, 121], [11C]4 (WC-98) , [18F]ICMT-11 , and [18F]WC-4-116 , are competitive caspase-3 inhibitors/labelers that can be traced using PET in order to detect caspase activation and apoptosis in vivo. However, a limitation exists in selectivity where other cysteine containing proteases can be labeled by these small molecule probes.
124. 29 [11C]-labeled N-benzyl-isatin sulfonamide analogues as PET tracers for apoptosis: synthesis, radiolabeling mechanism, and in vivo imaging study of apoptosis in Fas-treated mice using [11C]WC-98. Org Biomol Chem 7:1337–1348 Zhou D, Chu W, Rothfuss J, Zeng C, Xu J, Jones L et al (2006) Synthesis, radiolabeling, and in vivo evaluation of an 18F-labeled isatin analog for imaging caspase-3 activation in apoptosis. Bioorg Med Chem Lett 16: 5041–5046 Nguyen QD, Smith G, Glaser M, Perumal M, Arstad E, Aboagye EO (2009) Positron emission tomography imaging of drug-induced tumor apoptosis with a caspase-3/7 specific [18F]-labeled isatin sulfonamide.