By Afaf Kamal-Eldin, Jan Pokorny
Lipid oxidation, although researched because the starting of the 20 th century, nonetheless offers no whole and passable info at the composition of oxidized lipids. One very important issue contributing to those gaps in our wisdom approximately lipid oxidation pertains to the shortages in analytical technique. Analytical tools appropriate for oxidized lipids have been usually reviewed within the final decade, yet in general from the point of selection of person oxidized lipid sessions, resembling peroxides, aldehydes, polar lipids, or polymers. during this publication, they're handled from the viewpoint of varieties of analytical tools used, together with varied volumetric tools, UV-visible spectrometric equipment, excessive functionality size-exclusion chromatography, nuclear magnetic resonance spectroscopy, electron spin resonance spectroscopy, and differential scanning calorimetry. research of Lipid Oxidation is vital for extra advancements in analytical technique and hyphenated strategies, with which extra knowing of the response kinetics, mechanism, and implications will ensue.
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Additional resources for Analysis of lipid oxidation
Corps Gras 20, 10–26. Yu, T. O. (1957) 2-Thiobarbituric Acid Method for the Measurement of Rancidity in Fishery Products, Food Technol. 11, 104–108. Copyright © 2005 AOCS Press Ch4(OxiAnalysis)(40-69)Co1 3/23/05 8:49 PM Page 40 Chapter 4 Analysis of Nonvolatile Lipid Oxidation Compounds by High-Performance Size-Exclusion Chromatography Gloria Márquez-Ruiz and M. Carmen Dobarganes Instituto de la Grasa (CSIC), 41012 Sevilla, Spain Introduction High-performance size-exclusion chromatography (HPSEC) is a technique used to separate compounds according to their molecular size, normally related to their molecular weight (MW) provided that the compounds have a similar shape.
In the case of low PV, the results obtained by the two methods are practically the same, but at high PV, benzidine values are ~20% higher than the corresponding AV values (Pardun 1974). The anisidine method was modified with the use of a chloroform solvent system to facilitate the solubilization of solid samples (Jiros˘uová 1975). When this modification is used, the absorbance may be measured at 430 nm. The AV can be determined also by flow injection analysis using 2-propanol as a solvent and monitoring the absorbance continuously at 350 nm (Labrinea et al.
Supports of copolymer of styrene divinylbenzene were designed to operate across a wide spectrum of Copyright © 2005 AOCS Press Ch4(OxiAnalysis)(40-69)Co1 3/23/05 8:49 PM Page 42 solvents. Columns can even be transferred easily and rapidly between solvents of differing polarity without damage to the packed bed. Tetrahydrofuran (THF) is the most commonly used solvent, although toluene and dichloromethane are used for certain applications. 5 mL/min are the most usual, thus allowing performance of analysis in <30 min.