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By Harry Sobotka (Ed.), C.P. Stewart (Ed.)

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DETERMINATION OF $ 0 2 The determination of pCOz of blood by means of analyzing an air bubble brought into equilibrium with the blood sample was described by Riley et al. ( R l ) . The method was modified by Asmussen and Niel- 16 POUL ASTRUP AND 0. SIGGAARD-ANDERSEN sen (A1 ) in 1958. The technique is complicated and requires high technical skill, and it is not suitable for capillary blood. The principle of the pCOz eZectrode (Fig. 6 ) was described by Stow et al. (S17) in 1957, and later developed by Severinghaus and Bradley (S3) to a very sensitive electrode.

Arch. Blochem. Biophys. 38, 357-364 ( 1952). H1. Hasselbalch, K. , Die “reduzierte” und die “regulierte” Wasserstoffzahl des Blutes. Biochem. 2. 74, 56-62 (1916). J1. , Standard bicarbonate, its clinical significance, and a new method for its determination. Scand. J. Clin. Lab. Invest. 9, 122-132 (1957). L1. Lillienthal, J. , and Riley, R. , On the determination of arterial oxygen saturations from samples of “capillary” blood. J. Clin. Invest. 23, 904-906 (1944). L2. , and Riley, R. , On the estimation of arterial carbon dioxide from samples of cutaneous (capillary) blood.

002 pH units, which of course increases the absolute error of the blood pH measurement. 002 pH units. 2% for the apparatus described by Siggaard-Andersen and J@rgensen(S12). This means that the coefficient of variation for determination of the pC02 of blood is 2% (M2). If the found pC02 value is far from the pC02 of the gas mixture used for equilibration, the error is higher. 2 meq/l. A change in plasma protein concentra- 25 ACID-BASE VALUES OF BLOOD tion to pathological values and changes of some other factors of importance for the normal distribution of hydrogen ions between red cells and plasma will cause only small changes in the base-excess value, as determined by using the nomogram (S10).

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